Samples with high buffer capacity are not recommended to use at pH very different from neutral. There are no other restrictions. In the process of isolation, the sorbent with adsorbed DNA is washed from the impurities. The product can be used with a wide range of biosamples (buccal epithelium, human and bacterial cells, whole blood, blood plasma, etc), lysis reagents and detergents (guanidine, TRIS, Tween 20, Trithon x100, SDS, PEG, etc), organic solvents and molecular biology buffer solutions.

6–12 μg of genomic DNA can be isolated (from 200 μL of whole blood) per 1 mg of beads (= 0.04 ml of suspension with concentration of 25 mg/ml). 1.5 – 3 μg of genomic DNA can be isolated (from 200 μL of whole blood) per 1 isolation with ExtraMag kit (= 0.25 mg of the beads, = 0.01 ml of suspension with concentration of 25 mg/ml).

ExtraMag beads are intended for use in aqueous solutions. Any beads aggregate under certain conditions. You can test a free sample of ExtraMag beads to see how compatible they are with your buffer system. In most cases our beads show good results. If you want the best results, there are 2 possible solutions: -Use specially selected buffers, for example try any ExtraMag DNA/RNA Isolation Kit, based on the ExtraMag beads; -Try our new development - monodisperse spheres, which do not aggregate due to their shape. However, we would like to draw your attention to the fact that at this moment the product is at the testing stage.

Principally, the sorption of DNA onto magnetic particles is a reversible process. But we do not recommend reusing beads. Firstly, because of the possible contamination of a final product. Secondly, the surface of the particles can be clogged during use, and we cannot guarantee a good quality of the reused beads. If you require large quantities of beads, you can request a special offer for large volumes or regular purchases.

We can provide RUO ExtraMag kits for NA isolation in any package upon request. To clarify the conditions, please contact us.